Gene description for FLOT1
Gene name	flotillin 1
Gene symbol	FLOT1
Other names/aliases	-
Species	Homo sapiens
Experiment description of studies that identified FLOT1 in Breast cancer cells
1 Experiment ID 575 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Breast cancer cells Sample name BT549 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
2 Experiment ID 577 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Breast cancer cells Sample name MCF7 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
3 Experiment ID 578 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
4 Experiment ID 1230 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Western blotting PubMed ID 25503107    Organism Homo sapiens Experiment description Tumor-derived microvesicles mediate human breast cancer invasion through differentially glycosylated EMMPRIN. Authors Menck K, Scharf C, Bleckmann A, Dyck L, Rost U, Wenzel D, Dhople VM, Siam L, Pukrop T, Binder C, Klemm F. Journal name J Mol Cell Biol. Publication year 2015 Sample Breast cancer cells Sample name MCF-7 Isolation/purification methods Ultracentrifugation Filtration Density gradient centrifugation Flotation density 1.13-1.22 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140270: EV-METRIC:0%, 33%, 33%
5 Experiment ID 1231 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Western blotting PubMed ID 25503107    Organism Homo sapiens Experiment description Tumor-derived microvesicles mediate human breast cancer invasion through differentially glycosylated EMMPRIN. Authors Menck K, Scharf C, Bleckmann A, Dyck L, Rost U, Wenzel D, Dhople VM, Siam L, Pukrop T, Binder C, Klemm F. Journal name J Mol Cell Biol. Publication year 2015 Sample Breast cancer cells Sample name SK-BR-3 Isolation/purification methods Ultracentrifugation Filtration Density gradient centrifugation Flotation density 1.13-1.22 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140270: EV-METRIC:0%, 33%, 33%
6 Experiment ID 1678 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
7 Experiment ID 1681 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
8 Experiment ID 1682 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
9 Experiment ID 1683 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 4 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
10 Experiment ID 1684 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 5 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
11 Experiment ID 1685 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 6 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
12 Experiment ID 1998 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34112803    Organism Homo sapiens Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Authors Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. Journal name Nat Commun Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
13 Experiment ID 1999 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34112803    Organism Homo sapiens Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Authors Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. Journal name Nat Commun Publication year 2021 Sample Breast cancer cells Sample name MCF7 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
14 Experiment ID 2419 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 35939247    Organism Homo sapiens Experiment description Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems Authors Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS. Journal name Clin Exp Metastasis Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231T Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
15 Experiment ID 2420 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 35939247    Organism Homo sapiens Experiment description Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems Authors Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS. Journal name Clin Exp Metastasis Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231T Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
16 Experiment ID 2421 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 35939247    Organism Homo sapiens Experiment description Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems Authors Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS. Journal name Clin Exp Metastasis Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-435 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
17 Experiment ID 2422 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 35939247    Organism Homo sapiens Experiment description Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems Authors Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS. Journal name Clin Exp Metastasis Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-435 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
18 Experiment ID 2867 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 30918259    Organism Homo sapiens Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A Authors Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC. Journal name Nat Commun Publication year 2019 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
19 Experiment ID 2869 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 30918259    Organism Homo sapiens Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A Authors Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC. Journal name Nat Commun Publication year 2019 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
20 Experiment ID 2873 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 30918259    Organism Homo sapiens Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A Authors Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC. Journal name Nat Commun Publication year 2019 Sample Breast cancer cells Sample name MCF7 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
21 Experiment ID 2875 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 30918259    Organism Homo sapiens Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A Authors Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC. Journal name Nat Commun Publication year 2019 Sample Breast cancer cells Sample name MCF7 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
22 Experiment ID 2876 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 30918259    Organism Homo sapiens Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A Authors Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC. Journal name Nat Commun Publication year 2019 Sample Breast cancer cells Sample name MCF7 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
23 Experiment ID 3223 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32111843    Organism Homo sapiens Experiment description A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA Authors de Jong OG, Murphy DE, Mäger I, Willms E, Garcia-Guerra A, Gitz-Francois JJ, Lefferts J, Gupta D, Steenbeek SC, van Rheenen J, El Andaloussi S, Schiffelers RM, Wood MJA, Vader P. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Tangential flow filtration Filtration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
24 Experiment ID 3287 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
25 Experiment ID 3288 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
26 Experiment ID 3290 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 5 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
27 Experiment ID 3291 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 6 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
28 Experiment ID 3292 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 7 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
29 Experiment ID 3293 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 8 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
30 Experiment ID 3294 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 9 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
31 Experiment ID 3295 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 1 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density 1.05 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
32 Experiment ID 3296 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 3 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
33 Experiment ID 3297 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 4 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
34 Experiment ID 3298 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 5 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
35 Experiment ID 3299 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 6 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
36 Experiment ID 3300 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 7 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
37 Experiment ID 3301 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 8 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
38 Experiment ID 3302 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 - Fraction 9 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
39 Experiment ID 3303 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
40 Experiment ID 3304 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 32848136    Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S. Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
41 Experiment ID 3348 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry ELISA EV-TRACK -
42 Experiment ID 3359 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name LM2-4175 Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
43 Experiment ID 1664 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MCF7 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry EV-TRACK -
44 Experiment ID 1666 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry EV-TRACK -
45 Experiment ID 1678 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
46 Experiment ID 1678 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
47 Experiment ID 1679 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -